CZI Frontiers of Imaging Initiative – Cryo-CLEM Labels
Closing Date: 20/07/2023
Funding is available to support one-year collaborative research projects to accelerate the development of labeling probes for cryo-correlative light and electron microscopy (cryo-CLEM).
The Chan Zuckerberg Initiative (CZI) Frontiers of Imaging initiative, part of CZI’s broader Imaging programme, aims to drive the development of a suite of new imaging tools capable of observing biological processes across spatial scales at the level of tissues, cells, and proteins. To gain a deeper mechanistic understanding of biological systems, CZI’s Imaging programme aims to visualise and measure them across biological scales and in their biological context.
The Cryo-CLEM Labels grant programme aims to drive development of complete, general-purpose, intracellular imaging probes for cryo-correlative light and electron microscopy (cryo-CLEM). The long-term goal of this opportunity is to drive technology development aimed at visualising intracellular structure and function using cryo-ET. Proposals are requested that support the development of intracellular imaging probes that can withstand cryogenic preservation, provide high photon yields to allow accurate localisation in cryo-CLEM, and have sufficient electron contrast or a unique shape to be detectable in cryo tomograms.
Examples of potential areas within the scope of this Request for Applications include, but are not limited to:
- Complete solution for cryo-CLEM labeling that includes high-yield fluorophores and features that are detectable in electron microscopy (EM).
- Genetically encoded fluorescent and electron-dense tags with unique shapes for cryo-CLEM, ideally self assembling only when attached to protein of interest.
- Carbon nanodots or chemically synthesized probes adapted for cryo-CLEM.
- Nanobody binders with unique shapes for cryo-CLEM.
- AI and deep learning approaches to the design of cryo-CLEM labels.