CZI Frontiers of Imaging Initiative – Cryo-CLEM Labels

Closing Date: 20/07/2023

Funding is available to support one-year collaborative research projects to accelerate the development of labeling probes for cryo-correlative light and electron microscopy (cryo-CLEM).

The Chan Zuckerberg Initiative (CZI) Frontiers of Imaging initiative, part of CZI’s broader Imaging programme, aims to drive the development of a suite of new imaging tools capable of observing biological processes across spatial scales at the level of tissues, cells, and proteins. To gain a deeper mechanistic understanding of biological systems, CZI’s Imaging programme aims to visualise and measure them across biological scales and in their biological context.

The Cryo-CLEM Labels grant programme aims to drive development of complete, general-purpose, intracellular imaging probes for cryo-correlative light and electron microscopy (cryo-CLEM). The long-term goal of this opportunity is to drive technology development aimed at visualising intracellular structure and function using cryo-ET. Proposals are requested that support the development of intracellular imaging probes that can withstand cryogenic preservation, provide high photon yields to allow accurate localisation in cryo-CLEM, and have sufficient electron contrast or a unique shape to be detectable in cryo tomograms.

Examples of potential areas within the scope of this Request for Applications include, but are not limited to:

  • Complete solution for cryo-CLEM labeling that includes high-yield fluorophores and features that are detectable in electron microscopy (EM).
  • Genetically encoded fluorescent and electron-dense tags with unique shapes for cryo-CLEM, ideally self assembling only when attached to protein of interest.
  • Carbon nanodots or chemically synthesized probes adapted for cryo-CLEM.
  • Nanobody binders with unique shapes for cryo-CLEM.
  • AI and deep learning approaches to the design of cryo-CLEM labels.
Funding body Chan Zuckerberg Initiative (CZI)
Maximum value 165,000 USD
Reference ID S25273
Category Medical Research
Science and Technology
Engineering and Physical Sciences
Biotechnology and Biology
Fund or call Fund